Composite

Part:BBa_K4805008:Design

Designed by: Tianyin Zhao   Group: iGEM23_LINKS-China   (2023-10-12)


pTDH3-BmeTC_Y167A,D373C-tTDH1-pTEF2-ERG20_F96W-tSSA1-pPGK1-BmeTC-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1357
    Illegal NheI site found at 6331
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4112
    Illegal BglII site found at 4173
    Illegal BglII site found at 5638
    Illegal BamHI site found at 760
    Illegal BamHI site found at 5734
    Illegal XhoI site found at 2567
    Illegal XhoI site found at 4289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3828
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4275
    Illegal BsaI site found at 4536
    Illegal BsaI.rc site found at 4525
    Illegal BsaI.rc site found at 5648


Design Notes

composite


Source

composite

References